Light microscopy for biological samples

Course description:

The following topics are treated: perception, light, lenses, the microscope, brightfield microscopy, darkfield microscopy, phase contrast microscopy, (quantitative) polarised light microscopy, differential interference contrast microscopy, fluorescence microscopy, fluorescence recovery after photobleaching, Förster energy resonance transfer, total internal reflection fluorescence microscopy, laser scanning microscopy, confocal microscopy, spinning disk confocal microscopy, multi-photon excitation microscopy, second harmonic generation microscopy, (dual colour) fluorescence (cross) correlation spectroscopy, fluorescence lifetime imaging microscopy, single molecule localisation microscopy, photo-activated localisation microscopy, (rapid) (direct) stochastic optical reconstruction microscopy, stimulated emission depletion microscopy, ground state depletion microscopy (with individual molecule return), live cell imaging, electronic imaging.

Literature/study material: Handouts of lectures, Extra information handed out during the lectures. Students are expected to find their own way in literature (recommended)
Douglas B. Murphy & Michael W. Davidson; Fundamentals of light microscopy and electronic imaging (2nd edition); Wiley-Blackwell, October 2012; ISBN 978-0-471-69214-0 (recommended)

Registration: register at TU/e, course code 8MM10, at least 4 weeks before the start of the course. Please note that you also have to register for the exam, information can be found here.
UU Osiris registration will be done retroactively when the results from TU/e have been received. Please also inform us that you have applied for this course by sending an email to